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. 2014 May 5;9(5):e96699. doi: 10.1371/journal.pone.0096699

Figure 2. The frequency distribution of the area-size of P2X1–4 subunit-positive neurons.

Figure 2

(A), (B), (C), and (D) show P2X–IR sections that were counterstained with neutral red to precisely calculate the number of P2X1, P2X2, P2X3, and P2X4-positive neurons, respectively (using black positive cells compared with red negative cells). Scale bar in (D)  = 100 µm. (E), (F), (G), and (H) represent the frequency distribution of the area-size of P2X1, P2X2, P2X3, and P2X4-IR, respectively.