Table 1. Technical controls performed on mice with and without DDC treatment.

Sample	AB PLA m-a-K8	AB PLA gp-a-p62	PLA Probe PLUS	PLA Probe MINUS	PLA Detect. Kit	AB IIF rat-a-K8	Alexa 488 (a-rat)	Rhod. (a-gp)	Result
Standard diet	x	x	x	x	x	x	x		PLA and IIF positive
Standard diet	x	x	x	x	x				only PLA positive
Standard diet						x	x		only IIF positive
DDC diet	x	x	x	x	x	x	x		PLA and IIF positive
DDC diet	x	x	x	x	x				only PLA positive
DDC diet						x	x		only IIF positive
DDC diet	x		x	x	x		x		PLA and IIF negative
DDC diet		x	x	x	x		x		PLA and IIF negative
DDC diet		x				x	x	x	double IIF positive

In order to complement the biological controls (knockout mice) we performed a number of technical controls to make sure that no cross reactions between the different reagents might lead to false results.