Table 3. Prenyl transferase enzymatic assays with recombinant E. coli protein extracts.
| Strain Protein Extract | Variable Substrate | Prenyl Transferase Activity (U/mg×106) | Percentage of each Product* |
| BL21pET28 | DMAPP | - | ND |
| GPP | - | ND | |
| FPP | - | ND | |
| BL21+FPS | DMAPP | 6.06±0.75 | C10 (12.9%), C15 (87.1%) |
| GPP | 3.84±0.43 | C15 (100%) | |
| FPP | - | ND | |
| BL21+crtE | DMAPP | - | ND |
| GPP | - | ND | |
| FPP | 2.44±0.16 | C20 (100%) | |
| BL21+FPS and BL21+crtE | DMAPP | 6.65±0.11 | C10 (17.4%), C15 (71.5%), C20 (11.1%) |
Enzymatic assays were performed as described in the Materials and Methods section. Two protein extract samples from two replicate cultures were obtained for each strain, and then two enzymatic assays were performed with each protein extract. The table values are the mean values of prenyl transferase activity and percentage of product ± standard error of the mean.
*Percentage of each product considering 100% the total radioactivity found in the hexanic phase.
ND: Product not detected. C10, C15 and C20 correspond to products containing 10, 15 and 20 carbon atoms, respectively.