Figure 3. Poldip2 deletion increases Sirt1 expression and LC3b conversion but does not affect expression of senescence markers.

(A) p16^INK4a and (B) p19^ARF mRNA expression was compared between Poldip2+/+ (blue) and Poldip2−/− (red) cells by qRT-PCR and corrected for PPIA. (C) Sirt1 and (D) LC3b-1 and -II protein levels were assessed by western blot in Poldip2+/+ and Poldip2−/− MEFs at passages 2, 4 and 5 and normalized to β-actin. Error bars represent mean ± SEM of 3 independent experiments. * P<0.05 comparing Poldip2+/+ with Poldip2−/−.