Figure 7. Poldip2 activates the E2F1 pathway.

(A) Protein levels of E2F1 target genes were measured by immunoblot. Lysates from Poldip2+/+ and Poldip2−/− cells in passages 2, 4 and 5 were probed with antibodies against β-actin, Poldip2, E2F1, Cdk1 and PCNA. Densitometry was performed and corrected to β-actin (B–D). (E) Cdk1 mRNA levels were measured by RT-qPCR and corrected with PPIA. Error bars represent mean ± SEM of 3–4 independent experiments. * P<0.05 comparing Poldip2+/+ with Poldip2−/−.