Table 3. The effect of PakD on the mass and protein accumulation of cells.
Per 107 cells per hour | Per 107 nuclei per hour | ||||
Genotype | Mass (mg) | Protein (µg) | Nuclei, ×10−5 | Mass (mg) | Protein (µg) |
Wild type | 0.45±0.03 | 30±2 | 9.9±0.3 | 0.33±0.02 | 22±2 |
pakD– | 0.44±0.04 | 30±2 | 10.9±0.6 | 0.34±0.03 | 23±2 |
pakD–/actin15::pakD-GFP | 0.41±0.06 | 31±3 | 8.9±0.6 | 0.33±0.05 | 25±3 |
Mass and protein values from Table 2 were divided by the observed doubling time of the respective genotype. Doubling times were calculated as described in Materials and Methods. Values are the mean ± SEM from three or more independent experiments. Values for pakD– or pakD–/actin15::pakD-GFP were not significantly different from wild-type values or each other for any parameter shown (p>0.05, one-way ANOVA, Tukey's test).