Table 1. Histomorphological assessment of post-thaw isolated human islets in culture.

Variables		Culture duration	Pre-Edmonton protocol	Edmonton protocol	p value
Cumulative survival % measured in terms of islet mass in islet equivalents (IEQ)#		24 h	50.1 ± 10.6	69.4 ± 3.5	0.001
		3 d	17.3 ± 3.7	31.5 ± 1.6	0.001
		5 d	2.2 ± 0.5	11.9 ± 0.6	0.001
		7 d	0.1 ± 0	2 ± 0.1	0.001
In vitro tests	Viability (%)	24 h	8.3	59.1	0.001
		3 d	18.2	38.9	0.242
		5 d	66.7	81.0	0.353
		7 d	57.1	23.5	0.038
	Insulin stimulation index (SI) at cumulative survival of 50%	24 h	2.83	1.22	N/A
		3 d	N/A	1.12
Histomorphology – islet hormones	Insulin (%)	24 h	82.8 ± 10.5	82.9 ± 2.7	0.950
		3 d	89 ± 4.6	87.7 ± 1.4	0.428
		5 d	78.8 ± 5	84.2 ± 0.8	0.001
		7 d	73.2 ± 0.5	97 ± 0.3	0.001
		Overall	83.1 ± 9.1	86.9 ± 5.8	0.006
	Overall	Glucagon (%)	11.3 ± 3.8	10.7 ± 5.9	0.580
		PP(%)	5.8 ± 1.2	5.4 ± 1.7	0.142
		Somatostatin (%)	3.3 ± 1.6	3.5 ± 1.2	0.527
		Islet hormone ratios##	80: 11: 6: 3	82: 10: 5: 3	N/A
Histomorphology –tissue turnover	Apoptotic index (%)	24 h	2.7 ± 0.8	2.1 ± 0.7	0.315
		3 d	1.5 ± 0.6	1.5 ± 0.9	0.987
		5 d	0.8 ± 0.5	1.3 ± 0.8	0.443
		7 d	1 ± 1	0.4 ± 0.4	0.554
		Overall	1.8 ± 0.3	1.6 ± 0.3	0.572
	Necrotic index (%)	24 h	15 ± 13.8	12.5 ± 3.5	0.850
		3 d	8.4 ± 4.4	27.6 ± 4.5	0.030
		5 d	8.5 ± 3.5	5 ± 2.9	0.474
		7 d	15.6 ± 8.7	7.8 ± 1.3	0.342
		Overall	11.7 ± 4.3	12.3 ± 2.4	0.901
	Cell turnover (%)	24 h	-17.7 ± 13.1	-14.6 ± 4	0.808
		3 d	-10 ± 4.2	-29.1 ± 5.4	0.036
		5 d	-9.3 ± 3.6	-6.3 ± 2.9	0.542
		7 d	-16.5 ± 7.8	-8.2 ± 1.7	0.280
		Overall	-13.2 ± 4.1	-13.7 ± 2.6	0.926

^# Correlation estimates of islet equivalents and culture duration were r_s = -764, p < 0.001. Survival was manually computed. All values are in mean ± SE; ^##Islet hormone ratios, Insulin: Glucagon: PP: Somatostatin; Mitotic index was not computed as no mitosis was seen