Figure 6. Light microscopy images of young porcine endocrine pancreatic islets treated with the APO-BrdU IHC staining kit. 40× magnification. Islets cultured in TCPS plates with 0, 10 or 100 µM H₂O₂ compared with Time Zero (A) and islets embedded in fibrin with 0, 10, or 100 µM H₂O₂ compared with Time Zero (B) are shown. The percentage of apoptotic cells (brown) in the total cell population (blue and brown) is shown in Figure 6C. ’*’ represents a value of P < 0.05, ‘***’ represents a value of P < 0.001. *** compared Time Zero with T10 and Time Zero with T100 and each T-set compared with the corresponding F-set.