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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Xenotransplantation. 2013 Nov 5;21(1):72–83. doi: 10.1111/xen.12073

Figure 3. Expression of Gal and human proteins on genetically-modified pAEC.

Figure 3

(A) Flow cytometry was used to evaluate Gal and human transgene protein expression on human AEC in comparison to WT pAEC. Human AEC did not express Gal, but expressed high levels of CD46, CD55, CD141 (TBM), and CD201 (EPCR). In contrast, WT pAEC expressed Gal, but no human CD46, CD55, TBM, or EPCR. No GTKO pAEC expressed Gal. (B) In addition to lack of Gal expression, GTKO/CD46 pAEC expressed high levels of CD46, GTKO/CD46/CD55 pAEC expressed high levels of CD46 and CD55. GTKO/CD46/TBM pAEC expressed TBM at a level comparable to hAEC. Similarly, GTKO/CD46/EPCR pAEC expressed EPCR at a level comparable to hAEC.