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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Transfusion. 2013 Nov 5;54(6):1569–1579. doi: 10.1111/trf.12485

Fig. 1. Leukoreduction of RBC concentrates reduces platelet aggregation after exposure to stored supernatant and reduces accumulation of selected inflammatory mediators.

Fig. 1

RBCs were obtained from leukoreduced (LR) or nonleukoreduced (NLR) red cell concentrates stored for <8 days or >20 days. (A) Whole blood aggregation was quantified as maximum amplitude (ohms) following addition of stored RBCs to fresh PRP samples v/v for 10 minutes at 37°C and then activated with ADP (20 μM). Freshly prepared (FP) RBCs were used as a control. (B-D) Cell-free supernatant was isolated from stored RBC concentrates by centrifugation and analyzed by competitive enzyme immunoassay (EIA) for (B) sCD40L, (C) PGE2 and (D) TXB2. Mean +/− SEM, n=5, Statistical significance was determined by one-way ANOVA. *p<0.05, **p<0.01.