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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Transfusion. 2013 Nov 5;54(6):1569–1579. doi: 10.1111/trf.12485

Fig. 3. Supernatants from stored leukoreduced RBCs and 8-iso-PGF cause platelet activation and release of proinflammatory and prothrombotic mediators from platelets.

Fig. 3

(A-C) Freshly prepared PRP was isolated from two independent donors. PRP was incubated with stored RBC supernatants (Days 5, 19 and 40) from three independent donors for 2 hours. Cell-free plasma was collected by centrifugation and analyzed by immunoassay for TXB2 and sCD40L or by flow cytometry for CD62P expression. (D-E) Washed platelets were prepared and incubated with 8-iso-PGF (1-20 μM) or vehicle control (VEH). Results are displayed as fold change over VEH for three donors, where each donor is indicated by a different symbol. Mean +/− SEM, n=3-4 for each group. Statistical significance was determined by one-way ANOVA. *p<0.05, **p<0.01.