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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Transfusion. 2013 Nov 5;54(6):1569–1579. doi: 10.1111/trf.12485

Fig. 6. Hemoglobin has no effect on mediator release, but increases reactive oxygen species production in platelets.

Fig. 6

(A-C) Washed human platelets were treated for 2 hours at 37°C with hemoglobin in Adsol. (A) sCD40L, (B) PGE2 and (C) TXB2 levels in supernatants were measured by immunoassay. (D-E) ROS levels were measured in platelet suspsensions treated with Adsol (vehicle) or stored RBC supernatants (Days 12, 26 and 33) (D) or hemoglobin (E). Mean +/− SEM. Statistical significance was determined by one-way ANOVA. *p<0.05, **p<0.01.