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. 2014 Mar 6;306(9):G759–G768. doi: 10.1152/ajpgi.00018.2014

Fig. 1.

Fig. 1.

A: by immunofluorescence, HIBEpiC and cholagiocarcinoma (CCA) cell lines show immunoreactivity for NK1R. Negative controls using the blocking peptide for NK1R showed no reaction. Bar = 50 μm. B and C: evaluation of the expression of NK1R in CCA and nonmalignant cell lines by real-time PCR and immunoblots. The expression of NK1R was observed in nonmalignant cholangiocytes as well as in all the CCA cell lines used. Data are mean ± SE of 4 experiments. D: evaluation of the expression of NK1R, Tac1, and membrane metalloendopeptidase (MME) in CCA and nonmalignant cell lines by FACS analysis. The expression of NK1R and Tac1 increased, whereas the expression of MME decreased in CCA cell lines compared with HIBEpiC cells. Data are mean ± SE of 4 experiments. *P < 0.05 vs. HIBEpiC cells; **P < 0.05 vs. corresponding NK1R evaluations; #P < 0.05 vs. corresponding NK1R and Tac evaluations.