Fig. 2.

Protein abundance of Akt2, phosphorylated Akt at Ser⁴⁷³, forkhead box protein O1 (FOXO-1), and phosphorylated FOXO-1 at Thr²⁴ in singleton and twin fetuses in late gestation. A: Akt2 protein abundance tended to be lower (P = 0.06) in the PCUN and PIUN groups compared with controls in singletons. B, C, and D: the protein abundances of phosphorylated Akt (Ser⁴⁷³), FOXO-1, and phosphorylated FOXO-1 (Thr²⁴) were not different in either treatment group in singletons. E, F, and H: the protein abundances of Akt2, phosphorylated Akt (Ser⁴⁷³), and phosphorylated FOXO-1 (Thr²⁴) were higher in the PCUN and PIUN groups compared with controls in twins. G: FOXO-1 protein abundance was decreased only in PIUN group compared with controls in twins. Different letters denote significant differences between treatment groups.