Table 2.

Effects of PCUN and PIUN on the expression of candidate miRs in the liver in singleton and twin fetal sheep in late gestation

	Singletons			Twins			Predicted Target Protein
microRNA	Control [mean expression (reads/million)]	PCUN (fold change relative to controls)	PIUN (fold change relative to controls)	Control [mean expression (reads/million)]	PCUN (fold change relative to controls)	PIUN (fold change relative to controls)	Protein	nt match	No. of matches
hsa-miR-142-5p (−1 isomir)	195	×1.60↑	×1.99↑	250	×0.63↓	×0.60↓	IRS-2	7mer-1A	1
hsa-miR-493-3p	280		×1.66↑	436			p85	7mer-1A	1
hsa-miR-146b-5p	990	×1.87↑		1,061
hsa-miR-339-5p	10,779	×0.80↓	×0.80↓	7,818
hsa-miR-148a-3p	11,494		×0.76↓	11,592		×0.71↓
hsa-miR-19a-3p	13,193		×0.72↓	8,729
hsa-miR-19b-3p	28,477		×0.66↓	18,837
hsa-miR-30a-5p (+2 isomir)	41,451		×0.76↓	36,325			FOXO1	7mer-m8	1
							Cav-1	7mer-1A	1
hsa-miR-30e-5p (−4 isomir)	25,165		×0.80↓	21,641
hsa-miR-122-5p	117,180			140,513	×1.37↑	×0.77↓
hsa-let-7a-5p	3,304			5,709	×0.57↓	×0.57↓	IRS-2	8mer	1
							IR	8mer	1
							Akt2	8mer	1
hsa-let-7b-5p	1,966			2,742	×0.61↓	×0.61↓	IRS-2	8mer	1
							IR	8mer	1
							Akt2	8mer	1
hsa-miR-130a-3p	139			155	×0.63↓	×0.66↓
hsa-miR-16-2-3p (−4 isomir)	207			200	×0.40↓
hsa-miR-34c-5p	215			242	×0.57↓
hsa-miR-106b-5p	8,734			6,137		×1.68↑
hsa-let-7 g-5p (+1 isomir)	5,260			6,624		×0.62↓
hsa-miR-335-5p	6,079			6,865		×0.67↓
hsa-miR-379-3p (+3 isomir)	101			150		×0.61↓
hsa-miR-369-3p	483			518		×0.64↓
hsa-miR-34a-5p	595			560		×0.67↓
hsa-miR-382-5p	147			154		×0.64↓
hsa-miR-126-5p	11,447			10,243		×0.77↓

PCUN, periconceptional undernutrition; PIUN, preimplantation undernutrition; miR, microRNA; hsa denotes that data were mapped to human miRBase using the Applied Biosystems “Lifescope” software, representing sheep miR orthologs. ↑>1.5 with threshold of >100 reads/million or >1.2 with threshold of >10,000 reads/million; RSD <50%. ↓<0.67 with threshold of >100 reads/million or <0.83 with threshold of >10,000 reads/million; RSD <50%.