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Lipid and glucose metabolism following cytokine treatment of 3T3-L1 adipocytes. Nonesterified fatty acid (NEFA) efflux (A), perilipin abundance (B), NAD+/NADH (C), and 2-deoxyglucose uptake (D) were measured following 24-h treatment of adipocytes with 1 nM cytokine. In B, Western blot quantitation of perilipin protein signal was normalized to β-actin protein signal (n = 6). *P < 0.05 and **P < 0.01 compared with control.
