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. 2014 Mar 4;306(9):E1033–E1045. doi: 10.1152/ajpendo.00422.2013

Fig. 5.

Fig. 5.

Expression of factors linked to mitochondrial biogenesis and function in response to cytokine treatment. 3T3-L1 adipocytes were treated with 1 nM of the indicated cytokine for 24 h, and the mRNA (A) and protein (B and C) expression of factors linked to mitochondrial biogenesis and function were evaluated. B: Western blot analysis of adipocytes. C: quantitation of each protein was normalized to β-actin and graphed relative to control (n = 6). QPCR results were normalized to reference mRNA transcription factor IIE. In A, the graph of endothelial nitric oxide synthase (eNOS) expression has a broken y-axis so that the TNFα treatment group is visible. Tfam, transcription factor A mitochondria; NRF1, nuclear respiratory factor 1; PGC-1α, PPARγ coactivator-1α; Con, control; T, TNFα; 1β, IL-1β; 6, IL-6; Cyt c, cytochrome c; COX IV, cytochrome c oxidase subunit IV. *P < 0.05.