Fig. 2.

Increasing PKG activity attenuates renal fibrosis in a UUO mouse model. Representative light photomicrograph of the Masson staining of the kidney sections from 4 groups of WT and PKG-I Tg mice after 14 days of UUO or sham surgery (A) or from 2 groups of saline- and sildenafil-treated UUO mice (C). The positive collagen staining was shown as blue color. The scale bar represents 100 μm. Semiquantitative analysis was performed by calculating the positive area (B and D). Collagen type I (E–H) and collagen type III (I–L) mRNA and protein levels in kidney cortex from UUO or sham mice were determined by real-time PCR and Western blotting, respectively. Data are presented as means ± SE (n = 5–6 mice/group). *P < 0.05 vs. WT/sham group or saline group. #P < 0.05 vs. WT/UUO group. &P < 0.05 vs. Tg/sham group.