Fig. 3.

Increasing PKG activity affects kidney α-smooth muscle actin (SMA) and E-cadherin levels in a UUO mouse model. α-SMA (A–D) and E-cadherin (E–H) mRNA and protein levels in the kidney cortex from UUO or sham mice were determined by real-time PCR and Western blotting, respectively. Data are presented as means ± SE (n = 5–6 mice/group). *P < 0.05 vs. WT/sham group or saline group. #P < 0.05 vs. WT/UUO group. &P < 0.05 vs. Tg/sham group. I–J: kidney sections were stained with anti-α-SMA or E-cadherin antibody. The positive staining is shown as brown. Representative light micrographs are shown.