Fig. 7.

Increasing PKG activity reduced TGF-β1 production in the conditioned media (CM) from angiotensin II (ANG II)-treated macrophages or proximal tubular cells (PTC). A: macrophages were isolated from WT or PKG (Tg) mice. The expressions of PKG transgene and PKG activity (p-VASP) were determined by Western blotting. B–C: TGF-β1 protein levels in the CM from human ANG II (1 μM)-treated macrophages (MΦ) or PTC isolated from WT or PKG-I Tg mice were measured by ELISA. Data are presented as means ± SE (n = 3 individual experiments). ^P < 0.05 vs. WT MΦ. #P < 0.05 vs. WT/ANG II.