Fig. 3.

HG effect on receptor-operated Ca²⁺ entry (ROCE) in HMCs. Fura 2 fluorescence ratiometry shows SOCE and ROCE in HMCs with NG or HG treatment for 7 days. MCs were treated with 25 μM CPA for ∼7 min to deplete the internal Ca²⁺ stores. SOCE was estimated by the elevation of [Ca²⁺]_i upon switching of the bathing solution from a Ca²⁺-free to a 2 mM Ca²⁺ solution in the presence of CPA. ROCE was defined as the additional increase in [Ca²⁺]_i by adding ANG II (1 μM) in the presence of CPA. A–C: representative traces recorded ∼4 min after CPA application. [Ca²⁺]_B represents the Ca²⁺ concentration in the bathing solution. Thick arrows, application of ANG II with and without diltiazem (Dil) or SKF96365 (SKF) Traces were smoothed using SigmaPlot software (version 11.0). D: summary data averaged from NG groups with ANG II treatment (n = 29), HG with ANG II treatment (n = 18), NG with ANG II+Dil treatment (n = 16), and NG with ANG+SKF treatment (n = 15). *P < 0.05, compared with SOCE in NG group. †P < 0.01, compared with ROCE in NG group.