Fig. 4.

HG effect on STIM1 protein expression in HMCs. A: HMCs were cultured in 0.5% FBS NG media for 3 days or in 0.5% FBS HG media for different time periods. B: HMCs were cultured in 0.5% FBS media with different concentration of d-glucose for 7 days. Appropriate concentrations of α-mannitol were used as the osmotic control. C and D: summary data showing the time course and dose dependence of HG effect on STIM1 protein expression. In C, **P < 0.01 and *P < 0.05, NG vs. HG for the same time period. n, No. of independent Western blots. In D, *P ≤ 0.05, vs. 5.6 mM glucose.