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MMP1-KD cells migrate out of cell clusters. After 24 h of HGF treatment, control MDCK cell cysts have chains emerging from the cysts (A and B). MDCK cells with scrambled shRNA also show chains emerging from the cysts (C and D). At this time, MMP1-KD cells are able to migrate out of the cell clusters; however, not all cells are found in chains. Instead, many cells seem to migrate out of the clusters (E and F, arrowheads). Scale bars = 25 μm.
