Figure 4.

FSH stimulates several angiogenic processes in HUVECs with a similar efficacy as VEGF. Within a given experiment in each assay, serum-starved HUVECs were incubated with vehicle (no additions [NA]), FSH at 600 ng/mL final concentration, VEGF at 50 ng/mL final concentration, or LSGS, and the following assays were performed and quantified: tube formation (A), wound healing (B), cell migration (C), and cell proliferation as determined by Click-iT EdU (D). Data shown are the mean ± SEM of five, three, three, and four independent experiments in A, B, C, and D, respectively. Asterisks denote differences with P < .5.