Figure 6.

FSH stimulates Akt phosphorylation in HUVECs. Serum-starved HUVECs were incubated for the indicated times with FSH at 600 ng/mL final concentration or VEGF at 50 ng/mL final concentration. Total Akt (t-Akt) and phosphorylated Akt (p-Akt) were determined by Western blotting. A, Western blots from one representative experiment. B, The ratio of p-Akt/t-Akt was determined and plotted as the fold-increase relative to time zero. Data shown are the mean ± SEM of three independent experiments.