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Activation of PKA and mTOR occurs in the same cells in DRP-TpoKO tumors. Immunohistochemical staining of pCREB (A), pmTOR (B), and phospho-p70S6K (C) in DRP-TpoKO tumors is shown. IF staining of 4′,6′-diamino-2-phenylindole (DAPI; D), pCREB (E, red), pmTOR (F, green) in DRP-TpoKO tumors (G is the merged image of D–F). T, FTC; C, capsule. Arrow indicates capsular invasion. Scale bar (A, applies to B and C), 125 μm; (D, applies to E–G), 250 μm.
