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Evaluation of isoAsp detection methods.
| Method | Sample prep time (min) | Chromatography time (min) | Analysis of isoD only (min) | Location specific information |
|---|---|---|---|---|
| Peptide map | 150 | 140* | 10 | Yes |
| Focused peptide map | 150 | 30 | 5 | Yes |
| Digested-HIC | 30 | 40 | 5 | Domain specific |
Time includes separation gradient and a required cleaning gradient between each sample.
