Table 1.

Real-time quantitative PCR detection of syndapin isoforms in mouse adrenal medulla

	ΔCt	Relative Expression
Syndapin 1	7.76 ± 0.24	0.2
Syndapin 2	5.97 ± 0.30	0.9
Syndapin 3	5.82 ± 0.49	1.0

Adrenal medullae were obtained from adult mouse adrenal glands. Specific primers were used for real-time PCR detection of syndapin 1, 2, and 3 messages. ΔC_t values (means ± SE) represent the difference in cycle threshold (C_t) between expression of the housekeeping gene GAPDH and each syndapin isoform. Relative expression was calculated from mean C_t of each syndapin isoform, with syndapin 3 used as a reference. Data were pooled from 3 experiments, with each experiment consisting of 5 animals.