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Ca2+-free solutions, BAPTA-AM, and Tg reduce C12-induced caspase 3/7 in JME (A) and Calu-3 (B) cells. JME and Calu-3 cells were grown in plastic wells, exposed to Ca2+-containing (+Ca) or Ca2+-free (−Ca) solution or Ca2+-free solution containing BAPTA-AM (10 μM) for 30 min, treated with 50 μM C12 for 2 h, and then processed for caspase 3/7 activity. Values are means ± SD (n = 2–3 experiments). *P < 0.05 vs. Ctrl. +P < 0.05 vs. no Tg. #P < 0.05 vs. no BAPTA-AM.
