Fig. 2.
Dose dependence of Jag-1-mediated enhancement of SOCE in human PASMC. SOCE was induced by passive depletion of Ca2+ from the SR with 10 μM CPA in human PASMC. The active fragment (aa 188–204) of human Jag-1 protein was used for activation of Notch receptors. A: representative records showing the increases in [Ca2+]cyt due to SOCE in control PASMC (Vehicle control) and PASMC treated with 5, 50, or 500 μM Jag-1 (for 30 min). B: summarized data (means ± SE, n = 17–20 cells) showing the amplitude of the increase in [Ca2+]cyt due to Ca2+ release or leakage (left) and the amplitude of the peak (middle) and plateau (right) increases in [Ca2+]cyt in PASMC treated with 5, 50, or 500 μM Jag-1 (for 30 min). *P < 0.05, **P < 0.01 vs. control PASMC (0 μM) treated with vehicle (1% distilled water).