Fig. 3.

Inhibition of Notch signaling by the γ-secretase inhibitor DAPT attenuates Jag-1-mediated SOCE enhancement in human PASMC. SOCE was induced by passive depletion of Ca²⁺ from the SR with 10 μM CPA in human PASMC. The active fragment (aa 188–204) of human Jag-1 protein was used for activation of Notch receptors. A: representative records showing the increases in [Ca²⁺]_cyt due to SOCE in control PASMC and PASMC treated with scrambled Jag-1 (scJag-1, 50 μM for 30 min), Jag-1 (50 μM for 30 min), and Jag-1 (50 μM) plus DAPT (10 μM, an inhibitor of γ-secretase, for 30 min). B: summarized data (means ± SE, n = 17–20 cells) showing the amplitude of the increase in [Ca²⁺]_cyt due to Ca²⁺ release or leakage (Release, left) and the amplitude of the peak (middle) and plateau (right) increases in [Ca²⁺]_cyt in control PASMC and PASMC treated with scJag-1, Jag-1, or Jag-1 + DAPT (for 30 min). **P < 0.01 vs. vehicle control (Control, PASMC treated with 1% distilled water); ##P < 0.01 vs. Jag-1.