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. 2014 Feb 5;306(9):C819–C830. doi: 10.1152/ajpcell.00168.2013

Fig. 1.

Fig. 1.

Decreased migration of CFTR-silenced cells after electrical wounding. Cells were cultured on electric cell substrate impedance sensing (ECIS) arrays in serum-free media for 2 days before wounding experiments. A: immunoblot of CFTR expression in Calu-3 control and CFTR-silenced stable cell lines. Equal protein quantities (20 μg) were loaded per lane. Immunoblot of β-actin levels on the same blot is shown to demonstrate equal protein loading. B: phase micrographs of control and CFTR-silenced cells on ECIS arrays. Cells are shown on 250-μm diameter circular electrodes before wounding, immediately after wounding by acute electric shock, and 5 h after wounding. C: impedance tracings of control and CFTR-silenced cells over time after electrical wounding. Lines are averages of 4 replicate samples. D: T ½ values for recovery of impedance after cell wounding for control and CFTR-silenced cells. T ½ values represent the time at which impedance was ½ of the impedance reached at upper plateau. Values are means ± SE of 4 replicates. *P < 0.01, significantly different in two tailed t-tests.