FIG 3 .
Adenylated peptides from various sources inhibit E. coli AspRS in vitro. AspRS-catalyzed aminoacylation of tRNAAsp in S30 extracts prepared from wild-type E. coli was performed in the presence of aliquots of the indicated adenylation reaction mixtures with or without ATP. Extracts were incubated for 15 min to allow processing. The ratios of acid-insoluble radioactivity in the corresponding reaction mixtures with and without ATP are presented (mean values and standard deviations of measurements taken from three independently performed experiments are shown).