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Esx-3 components contribute to low-iron growth. The growth of the fxbA and fxbA esx-3 M. smegmatis mutants in low-iron medium was monitored by determining their optical densities at 600 nm. pJ, pJEB402 vector; pJ-[gene name], pJEB402 containing the indicated M. smegmatis gene. The experiments were performed 2 to 6 times in triplicate. Representative data are shown, and error bars represent the standard deviations from the replicates.
