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. 2014 May 5;211(5):801–814. doi: 10.1084/jem.20131780

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© 2014 O’Donovan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Expression of kaB-RAF substantially rescues sensory afferent growth in the absence of TrkA/NGF signaling. (A, left) Normal sensory cutaneous innervation at E16.5. (middle) Sensory cutaneous innervation is lost in embryos lacking the NGF receptor TrkA. (right) Expression of kaB-RAF restores cutaneous innervation. Arrowheads label the blue β-gal–positive (presumptive TrkA⁺) sensory trajectories. (B) Visualization of axon growth patterns after tissue clearing. The thoracic somatosensory innervation driven by kaB-RAF in a TrkA^−/− embryo (bottom; compare with middle for TrkA^−/− alone) is similar to that seen in a control TrkA^WT/− littermate (top). White arrowheads indicate the normal pathways of peripheral axons extending from thoracic DRGs. Red arrowheads indicate sensory projections rescued by kaB-RAF in the TrkA^−/− background. (C) Expression of kaB-RAF substantially rescues trigeminal TrkA⁺ afferent growth in the absence of TrkA/NGF signaling. Presumptive TrkA⁺ trigeminal axon projections (top) are lost in TrkA-deficient mice (middle) and are rescued by kaB-RAF (bottom). Ga, great auricular nerve; Go, greater occipital nerve; Mn, mandibular branch; Mx, maxillary branch; Op, ophthalmical branch. Images show littermates and are representative of three embryos per genotype. Bars: (A) 2 mm; (B and C) 1 mm.