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. 2014 May 5;211(5):943–959. doi: 10.1084/jem.20130790

Figure 3.

Figure 3.

RGMb expression in mouse macrophages and RGMb protein structure. (a) Flow cytometric analysis of cell surface expression of mRGMb and mPD-L2 on indicated cells using PE-conjugated mAbs. (b and c) RGMb expression in the indicated cell type by Western blotting using mAb 1H6. The loading amount was 60–80 µg/lane except for 0.5 or 1 µg/lane for 300-mRGMb. MØ indicates macrophages. Thio-MØ indicates thioglycollate-induced peritoneal macrophages. (d) Model of RGMb protein structure. Data in a–c are representative of two or more experiments.