Table 1. Bacterial strains and plasmids used in this study. KmR, ApR, and CmR denotes kanamycin, ampicillin, and chloramphenicol resistance, respectively.
| Strains or plasmids | Description | Source |
|---|---|---|
| E. coli K-12 | ||
| BTH101 | F−, cya-99 araD139 galE15 galK16 rpsL1 (Str r) hsdR2 mcrA1 mcrB1 | (Gully and Bouveret, 2006) |
| BW25113 | rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 | (Baba et al., 2006) |
| BW25113 ΔghoS | BW25113 ΔghoS Ω KmR | (Baba et al., 2006) |
| BW25113 ΔghoS ΔmqsRAΩKmR | BW25113 ΔghoS::frt ΔmqsRA Ω KmR | (Wang et al., 2013) |
| BW25113 ΔghoS ΔmqsRA ΔKmR | BW25113 ΔghoS::frt ΔmqsRA::frt | This study |
| BW25113 ΔghoT | BW25113 ΔghoT Ω KmR | (Baba et al., 2006) |
| BW25113 ΔmqsRA | BW25113 ΔmqsRA ΔKmR | (Kim et al., 2010) |
| Plasmids | ||
| pBAD-mqsA | ApR, pBR322 ori, PARA::mqsA-flag+ | This study |
| pBAD-arA | ApR, pBR322 ori, PARA::arA-flag+ | This study |
| pBAD-myc-His B | ApR, pBR322 ori, PARA::myc-His6+ | Life Tech. |
| pBR-plac | ApR, pBR322 ori, PLlacO-1 | (Guillier and Gottesman, 2006) |
| pBR-plac-toxI | ApR, pBR322 ori, PLlacO-1::toxI+ | This study |
| pBS(Kan)-mqsA | KmR; Plac::mqsA | (Kim et al., 2010) |
| pCA24N | CmR; lacIq | (Kitagawa et al., 2005) |
| pCA24N-arA | CmR; lacIq PT5-lac::arA-flag+ with two mqsRA palindromes between lacO and RBS | This study |
| pCA24N-arT | CmR; lacIq PT5-lac::His6-arT+ with two mqsRA palindromes between lacO and RBS | This study |
| pCA24N-arT-arA | CmR; lacIq PT5-lac::His6-arT+, arA-flag+ with two mqsRA palindromes between lacO and RBS | This study |
| pCA24N-arT (GFP+) | CmR; lacIq PT5-lac::His6-arT-gfp+ | This study |
| pCA24N-ghoS | CmR; lacIq PT5-lac::His6-ghoS+ | (Kitagawa et al., 2005) |
| pCA24N-ghoS(GFP+) | CmR; lacIq PT5-lac::His6-ghoS-gfp+ | (Kitagawa et al., 2005) |
| pCA24N-ghoS-L25I | CmR; lacIq PT5-lac::His6-ghoS+ with TTA (121-123 nt relative to start codon) mutated to ATA | This study |
| pCA24N-ghoS-M31L | CmR; lacIq PT5-lac::His6-ghoS+ with ATG (139-141 nt relative to start codon) mutated to TTG | This study |
| pCA24N-ghoS-M31L-mqsA | CmR; lacIq PT5-lac::His6-ghoS+-M31L-mqsA-flag+ | This study |
| pCA24N-ghoT | CmR; lacIq PT5-lac::His6-ghoT+ | (Kitagawa et al., 2005) |
| pCA24N-ghoT(GFP+) | CmR; lacIq PT5-lac::His6-ghoT-gfp+ | (Kitagawa et al., 2005) |
| pCA24N-mqsR | CmR; lacIq PT5-lac::His6-mqsR+ | (Kitagawa et al., 2005) |
| pCA24N-toxI-arT | CmR; lacIq PT5-lac::His6-arT+, PLlacO-1::toxI+ | This study |
| pCP20 | ApR, CmR; FLP+, λ cI857+, λ pR Repts | (Cherepanov and Wackernagel, 1995) |
| pKT25linker | KmR, p15A ori, Plac::T25+ | (Gully and Bouveret, 2006) |
| pKT25linker-ghoS | KmR, p15A ori, Plac::T25-ghoS+ | This study |
| pKT25linker-arT | KmR, p15A ori, Plac::T25-arT+ | This study |
| pKT25linker-zip | KmR, p15A ori, Plac::T25-zip+ | (Gully and Bouveret, 2006) |
| pUT18Clinker | ApR, ColE1 ori, Plac::T18+ | (Gully and Bouveret, 2006) |
| pUT18Clinker-mqsA | ApR, ColE1 ori, Plac::T18-mqsA+ | This study |
| pUC18Clinker-arA | ApR, ColE1 ori, Plac::T18-arA+ | This study |
| pUT18Clinker-zip | ApR, ColE1 ori, Plac::T18-zip+ | (Gully and Bouveret, 2006) |
arA refers to mqsA with TTC (64-66 nt relative to start codon) mutated to CTC, TGT (109-111 nt relative to start codon) mutated to AGT, GAT (151-153 nt relative to start codon) mutated to AAT, TTT (178-180 nt relative to start codon) mutated to CTT. arT refers to ghoS with TTA (121-123 nt relative to start codon) mutated to ATA, and ATG (139-141 nt relative to start codon) mutated to TTG.