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Antagonistic effect of MraW on MraZ. (A) Spot dilution assay of WT MG1655 containing the pDSW208 vector alone, pDSW208-mraZ, or pDSW208-mraZ-mraW on LB plates containing 1 mM IPTG to fully induce gene expression. Shown from left to right are undiluted and 10−1 to 10−5 diluted samples of a mid-logarithmic-phase culture grown in LB with no induction. (B) Growth curves of WT MG1655 and the mraW null mutant in LB without IPTG containing either the pDSW208 vector alone (V) or pDSW208-mraZ.
