FIG 3.

MraZ localizes to the nucleoid. WT MG1655 containing an mraZ-gfp fusion in pDSW208 was grown in LB to an OD₆₀₀ of ∼0.2 with no IPTG induction. Cells were moderately inhibited for septation even under these uninduced conditions because of leaky expression from the plasmid trc promoter. Differential interference contrast (DIC), DAPI-stained, MraZ-GFP fluorescence, and merged DAPI-GFP images of a representative dividing filament are shown (scale bar, 3 μm).