FIG 7.

Gene expression analysis by RNA-seq. The numbers of genes activated (white columns) or repressed (black columns) by MraZ are shown; they were binned by the fold changes in their expression (top). These genes were selected as follows. First, only genes with an FDR of ≤0.05 were chosen; second, the cutoff for differential gene expression was set at a nonlogFC value of ≥2 (or ≥2-fold). (A) WT MG1655 and a mraZ mutant containing the pDSW208 vector were grown in LB to stationary phase (OD of ≥1.4). Cells were collected, and the total RNA was extracted and subjected to RNA-seq analysis. (B) WT MG1655 containing either the pDSW208 vector alone or carrying mraZ was grown in LB to early log phase (OD of ≤0.1) and induced with 50 μM IPTG for 30 min. Cells were collected, and total RNA was extracted and subject to RNA-seq analysis.