FIG 1.

SearchDOGS Bacteria's pillar generation method. A set of initial pillars (highlighted in green) is created using reciprocal best BLASTP results. The orthology of genes producing one-way BLASTP hits is confirmed by automatically searching for shared synteny. In the example shown, the gene from species 1 highlighted in blue has neighboring genes that are in ortholog pillars with neighboring genes of the gene from species 2, confirming that the species 1 gene in blue and the species 2 gene beneath it belong in a single ortholog pillar.