FIG 9.

Nutrient germination of spoVAEa or spoVAF mutant spores with or without ectopic expression of spoVAEa and spoVAF. Heat-activated spores of various strains were germinated with l-valine, and DPA release was measured by determining Tb-DPA fluorescence as described in Materials and Methods. Results are presented in relative fluorescence units (RFU). Numbers of RFU of spores of different strains were corrected for the amounts of DPA in different spore preparations. Symbols: ○, PS832 (wild type); ●, PS4348 (spoVAEa mutant); △, PS4351 (spoVAF mutant); □, PS4391 (spoVAEa PsspB-spoVAEa-spoVAF mutant); ▲, PS4392 (spoVAF PsspB-spoVAEa-spoVAF mutant).