TABLE 2.
Kinetic parameters of the l-valine germination of wild-type and spoVAEa mutant sporesa
| Spores | Tlag (min) | Trelease (min) | ΔTrelease (min) | ΔTlys (min) | Ilag | Irelease |
|---|---|---|---|---|---|---|
| PS533 (wild type) | 21 ± 14b | 25 ± 14 | 3.8 ± 1.2 | 7 ± 4 | 0.81 ± 0.08 | 0.21 ± 0.07 |
| PS4348 (spoVAEa) | 45 ± 18c | 49 ± 19 | 4.9 ± 1.8 | 10 ± 6 | 0.60 ± 0.09 | 0.16 ± 0.07 |
a
The germination of individual spores with l-valine was analyzed as described in Materials and Methods, and kinetic parameter values were determined as described previously and as indicated in Fig. 7B and C.
b
Spores were observed for 90 min, and the data are average values and standard deviations from analyses of ≥80 spores that germinated.
c
This value is a significant underestimate of the Tlag value for the entire spoVAEa mutant spore population, since only 74% of the spores examined germinated during the 90-min observation period while 97% of the wild-type spores did so.