FIG 7.

RNA-seq reveals new Dot/Icm substrates. Cytosolic levels of cAMP were measured following infection of THP-1 macrophages for 2 days with wild-type C. burnetii or the ΔdotA mutant expressing CyaA alone or CyaA fused to possible Dot/Icm substrates. Elevated levels of cAMP indicating secretion were observed for CBU0122, CBU1530, CBU1614, CBU1685, CBU1686, and CBU1752. Results shown are from one experiment conducted in duplicate and are representative of three independent experiments. Error bars indicate the standard deviations from the means.