TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| E. coli Stellar | F− endA1 supE44 thi-1 recA1 relA1 gyrA96 phoA ϕ80ΔlacZΔM15 Δ(lacZYA-argF)U169 Δ(mrr-hsdRMS-mcrBC) ΔmcrA λ− | Clontech |
| E. coli PIR1 | F− Δlac-169 rpoS(Am) robA1 creC510 hsdR514 endA recA1 uidA(ΔMluI)::pir-116 | Invitrogen |
| C. burnetii Nine Mile (RSA439) | Phase II, clone 4 | Beare et al. (12) |
| C. burnetii ΔpmrA | Nine Mile, phase II containing a pmrA (cbu1227) deletion; Kanr | This study |
| C. burnetii ΔdotA | Nine Mile, phase II containing a dotA (cbu1648) deletion; Kanr | Beare et al. (12) |
| Plasmids | ||
| pJB-Kan | pJB2581 containing kan driven by P1169; Kanr | Omsland et al. (24) |
| pJC-CAT | pJC84 containing cat driven by P1169; Cmr | Beare et al. (12) |
| pJC-CAT::cbu1227-5′3′ | 5′- and 3′-end-flanking DNA from cbu1227 cloned into pJC-CAT; Cmr | This study |
| pJC-CAT::cbu1227-5′3′-Kan | P1169-Kan cassette cloned into pJC-CAT::cbu1227-5′3′; Cmr Kanr | This study |
| pTnS2::P1169-tnsABCD | cbu1169 promoter cloned into pTnS2; Ampr R6K ori | Beare et al. (12) |
| pMiniTn7T-CAT | P1169-CAT cloned into pUC18R6K-mini-Tn7T-Gm; Cmr Ampr R6K ori | Beare et al. (12) |
| pMiniTn7T-CAT::cbu1227/1228comp | cbu1227/1228comp fragment cloned into pMiniTn7T-CAT; Cmr Ampr | This study |
| pGSV4 | Vector containing the luxCDABE operon | Warawa et al. (45) |
| pMiniTn7T-CAT::luxCDABE | Promoterless luxCDABE fragment cloned into pMiniTn7-CAT; Cmr Ampr | This study |
| pMiniTn7T-CAT::P0021-luxCDABE | cbu0021 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P0794-luxCDABE | cbu0794 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P0814-luxCDABE | cbu0814 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PenhC-luxCDABE | enhC promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P1213-luxCDABE | cbu1213 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P1226-luxCDABE | cbu1226 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PpmrA-luxCDABE | pmrA promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P1314-luxCDABE | cbu1314 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PgltA-luxCDABE | gltA promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P1556-luxCDABE | cbu1556 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P1636-luxCDABE | cbu1636 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PrpoS-luxCDABE | rpoS promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P1751-luxCDABE | cbu1751 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P1758-luxCDABE | cbu1758 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P1823-luxCDABE | cbu1823 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::P2052-luxCDABE | cbu2052 promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PicmH-luxCDABE | icmH promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PicmW-luxCDABE | icmW promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PicmV-luxCDABE | icmV promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PdotD-luxCDABE | dotD promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PicmT-luxCDABE | icmT promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PcoxigA-luxCDABE | CoxigA gene promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pMiniTn7T-CAT::PicmD-luxCDABE | icmD promoter cloned into pMiniTn7T-CAT-luxCDABE | This study |
| pJB-CAT-CyaA | C. burnetii CyaA fusion vector; Cmr Ampr | Beare et al. (12) |
| pJB-CAT-CyaA-cbu0122 | cbu0122 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu0409 | cbu0409 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu0508 | cbu0508 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu0705 | cbu0705 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu1231 | cbu1231 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu1530 | cbu1530 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu1540 | cbu1540 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu1614 | cbu1614 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu1651 | cbu1651 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu1685 | cbu1685 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu1686 | cbu1686 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cbu1752 | cbu1752 cloned into pJB-CAT-CyaA | This study |
| pJB-CAT-CyaA-cpeD | cbuA0015 cloned into pJB-CAT-CyaA | Beare et al. (12) |
| pJB-CAT-CyaA-cpeE | cbuA0016 cloned into pJB-CAT-CyaA | Beare et al. (12) |