TABLE 1.
Bacterial strains, plasmids, and oligonucleotides used in this study
| Strain, plasmid, or oligonucleotide | Description or sequence (5′–3′)a | Reference or source |
|---|---|---|
| Strains | ||
| C. jejuni DRH212 | Streptomycin-resistant C. jejuni 81-176 (Smr) | 10 |
| C. jejuni ΔrpoN | rpoN deletion mutant of C. jejuni DRH212 (Smr) | 10 |
| C. jejuni kpsM::kan | kpsM insertion mutant of C. jejuni 81-176 | 15 |
| C. jejuni mapA::kan | mapA insertion mutant of C. jejuni DRH212 (Knr Smr) | This study |
| E. coli DH5α | E. coli strain used for cloning | Invitrogen |
| Plasmids | ||
| pRY109 | Campylobacter coli cat cassette plasmid (Cmr Apr) | 26 |
| pGEM-T Easy | Subcloning vector (Apr) | Promega |
| pKinnick | Plasmid containing hawkeye transposon (Cmr Apr) | This study |
| Oligonucleotides | ||
| 5′cat_INSeq | ACGCGTCCTAACAGGTTGGATGATAAGTCCCCGGTCTTCGTATGCCGTCTTCTGCTTGGCGCGCCCTCGAGCAATTGTGCTCGGCGGTGTTCCTTTCCAA | |
| 3′cat_INSeq | ACGCGTCCTAACAGGTTGGATGATAAGTCCCCGGTCTTCGTATGCCGTCTTCTGCTTGGCGCGCCCTGCAGTCTAGTGCGCCCTTTAGTTCCTAAAGGGT | |
| JJ17 | CTAGCGAGCTTCCTCCTGTT | |
| JJ18 | CATCTCTTGCGTCAGGCAAA | |
| JJ19 | GGATCCCCGCATTAAAATTCACATCAAC | |
| JJ20 | GGATCCGCTAGAGGAATAGTTGTGCTT |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Smr, streptomycin resistance.