FIG 5.

Transconjugant Methylobacterium strains containing pJM10 vary in their sensitivity to high external concentrations of NaCl (A) and to low external pH (B). Cultures were grown in M-PIPES containing succinate as the carbon and energy source. Growth rates were measured in 48-well plates using an automated system. M. nodulans grows as a biofilm at 150 mM NaCl, making growth rates difficult to measure. Error bars show one standard deviation, calculated from three biological replicates. The experiment was repeated three times, with similar results.