TABLE 1.
Strains and primers used in this study
| Strain or primer | Relevant characteristic(s) or sequence (5′–3′)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ− thi-1 gyrA96 relA1 | Stratagene |
| BL21-CodonPlus(DE3)–RIL | E. coli B F− ompT hsdS(rB− mB−) dcm+ Tetr gal λ(DE3) endA Hte [argU ileY leuW; Camr] | Agilent Technologies |
| T. kodakarensis | ||
| KU216 | ΔpyrF | 34 |
| DAD | ΔpdaD ΔpyrF | 32 |
| DBP1 | ΔpdaD ΔbpsA::pdaD ΔpyrF | This study |
| Primers | ||
| tk0545-Fw | GGAAAACCATATGATGGCTGGAAAGGTCAG | This study |
| tk0545-Rv | GGAATTCTCAGAAGACGTTTACCTTGTCCT | This study |
| tk0548-Fw | GGAAAACCATATGATGGCGCTGAGCGACAG | This study |
| tk0548-Rv | GGAATTCTTAAACGAGCTTTTTCTCCTTCA | This study |
| tk0967-Fw | AAAAAAACATATGATGAGGATCGAAAGGCTGAA | This study |
| tk0967-Rv | AGAATTCTCAAATAAGCTCCCTCTCCG | This study |
| tk1691-Fw | AAAAAAACATATGATGAGGGAGATAATTGAGAG | This study |
| tk1691-Rv | AGAATTCTCAGGTAGTCGAGCTCTCCT | This study |
| tk1691-up1000-Fw | TTCCCCTTCTCATCGACATC | This study |
| tk1691-down1000-Rv | AATCTAGAACGTCTCCCAGATCAGC | This study |
| tkpdaD-Fw1 | AAGGATCCCGAGAATGATGTTTTAGC | This study |
| tkpdaD-Rv2 | GACTAGTTCAGTAGGGGAACATGAC | This study |
| inv-TK1691-Fw | GACTAGTGCCTTTCTGATTTATTTT | This study |
| inv-TK1691-Rv | AAGGATCCATCTCACACCTCCAGAAG | This study |
| tk1691_out_1 | GTTCTTATTTTTTTGTTTGT | This study |
| tk1691_out_2 | AAAAAAAATTAATTAGCCACGCACCCCCTAGGG | This study |
| tk1691_in_1 | GAGGCTCGCGAAGAAGAAGG | This study |
| tk1691_in_1 | ACGAATATCGCGCCCTCCTC | This study |
a
Underlined sequences indicate restriction enzyme sites.