TABLE 1.
Mycobacterial strains and plasmids used in the study
| Strain or plasmid | Relevant characteristic(s) | Source |
|---|---|---|
| Mycobacterial strains | ||
| M. tuberculosis H37Rv | Type strain | VLA Weybridge collection |
| M. tuberculosis ΔhupB | hupB knockout strain of M. tuberculosis H37Rv | This study |
| M. tuberculosis ΔhupB/pMS101 | M. tuberculosis ΔhupB complemented with pMS101 plasmid (see below) | This study |
| Plasmids | ||
| pSMT100 | Mycobacterial suicide vector utilized to create mutant strain based on homologous recombination | VLA Weybridge collection |
| pMS1 | 1,074-bp left-flanking region cloned into pSMT100 | This study |
| pMS2 | 1,048-bp right-flanking region cloned into pMS1 to generate pMS2 containing both the left- and right-flanking regions of hupB | This study |
| pSM96 | Mycobacterial high-level expression vector with Kanr and Hygr; contains a strong dnaK mycobacterial promoter upstream of multiple cloning site | VLA Weybridge collection |
| pMS101 | hupB of M. tuberculosis cloned into pSM96 | This study |