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. 2014 May;196(10):1853–1865. doi: 10.1128/JB.01483-13

TABLE 2.

Genes influenced by iron and HupB

Gene and function Locus no. Fold change in transcript levela
 Gene productd
M. tuberculosis LI versus HIb P valuec LI M. tuberculosis ΔhupB versus LI M. tuberculosis P valuec
Mycobactin biosynthesis and iron storage
    mbtI Rv2386c 5.7123 0.0001 −2.9955 0.0014 Isochorismate synthase MbtI
    mbtD Rv2381c 4.6057 0.0000 −3.0568 0.0001 Polyketide synthetase MbtD
    mbtB Rv2383c 4.5971 0.0000 −3.2997 0.0000 Phenyloxazoline synthase MbtB
    mbtC Rv2382c 4.4677 0.0000 −3.2378 0.0000 Polyketide synthetase MbtC
    mbtE Rv2380c 4.2260 0.0000 −2.5399 0.0007 Peptide synthetase MbtE
    mbtG Rv2378c 4.1033 0.0028 −2.1138 0.0045 Lysine-N-oxygenase MbtG
    mbtA Rv2384 4.0104 0.0001 −2.2350 0.0295 Bifunctional enzyme MbtA
    mbtH Rv2377c 3.8216 0.0000 −2.2790 0.0001 Putative conserved protein MbtH
    mbtF Rv2379c 2.9341 0.0007 −1.9214 0.0008 Peptide synthetase MbtF
    bfrB Rv3841 −4.3759 0.0002 2.1293 0.0008 Bacterioferritin BfrB
    bfrAe Rv1876 −1.1001 0.0187 1.0505 0.0473 Bacterioferritin BfrA
Other
    esxR Rv3019c 1.7064 0.0003 −3.3496 0.0001 Secreted ESAT-6-like protein
    hisE Rv2122c 4.4240 0.0000 −3.2940 0.0012 AMP pyrophosphatase HisE
    rv0678 Rv0678 1.1304 0.0115 −3.2729 0.0012 CHP
    mmpS5 Rv0677c 1.7504 0.0119 −3.2452 0.0010 CMP, MmpS5
    mmpL5 Rv0676c 2.7267 0.0000 −3.1554 0.0000 CTMP
    rv3402c Rv3402c 4.3656 0.0001 −3.0883 0.0005 CHP
    ppe4 Rv0286 1.8776 0.0208 −3.0091 0.0044 PPE family protein
    eccD3 Rv0290 1.9662 0.0013 −2.9816 0.0004 ESX conserved component EccD3; ESX-3 type VII secretion system protein
    rv3839 Rv3839 4.5804 0.0000 −2.8439 0.0001 CHP
    mycP3 Rv0291 1.9005 0.0088 −2.7333 0.0021 Mycosin MycP3
    espG3 Rv0289 1.7913 0.0167 −2.7083 0.0088 ESX-3 secretion-associated protein EspG3
    rv2028c Rv2028c 1.5179 0.0004 −2.6419 0.0002 Universal stress protein family protein
    rv2030c Rv2030c 1.1573 0.0043 −2.5301 0.0000 CHP
    ppe37 Rv2123 4.5183 0.0000 −2.5139 0.0023 PPE family protein
    irtA Rv1348 3.8448 0.0000 −2.3819 0.0001 Iron-regulated transporter IrtA
    irtB Rv1349 3.7224 0.0000 −2.2015 0.0001 Iron-regulated transporter IrtB
a

Fold changes of ≥2 in transcript levels were considered.

b

HI and LI represent high (8 μg Fe ml−1)- and low (0.02 μg Fe ml−1)-iron conditions of growth.

c

The P values were calculated based on multiple probes used for each gene using the t test method.

d

Gene products are indicated as described by Mycobacterial Browser (Mycobrowser; Tuberculist version 2.6; http://tuberculist.epfl.ch/), except that the terms “probable” and “possible” are removed. CHP, conserved hypothetical protein; CMP, conserved membrane protein; CTMP, conserved transmembrane protein.

e

This gene is included even though the fold change is <2.